cd1 pregnant female mice  (Charles River Laboratories)

Journal: Frontiers in Pharmacology

Article Title: Impact of in-utero electronic cigarette exposure on neonatal neuroinflammation, oxidative stress and mitochondrial function

doi: 10.3389/fphar.2023.1227145

Figure Lengend Snippet: Study design: pregnant CD1 mice were exposed to e-cig (Blu, 2.4% nicotine) from gestational day 5 (E5) to postnatal day 7 (PD7), 6 times per day following the smoking protocol CORESTA. In between this exposure length, a group of pregnant mice ( n = 3) were sacrificed to isolate the primary neurons from the pups. Rest of the pregnant moms were continued to exposure till PD7. At PD7, another group of offspring were sacrificed ( n = 10) and isolated the brain. Rest of the offspring were continued to grow till PD90 and another group of both male and female offspring were sacrificed and brains were isolated. Experiments were performed in primary neuron and brain tissue lysate at PD7 and PD90.

Article Snippet: Female CD1 pregnant mice (Charles River Laboratories, Inc., Wilmington, MA; Cat# CRL:22, RRID: IMSR_CRL:22) were kept under standardized light and dark conditions (12 h), humidity (70%) and temperature (22°C) after delivery their offspring.

Techniques: Isolation